PCR working principle| PCR Workstation Manufacturer and Supplier

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PCR working principle:

A technique that is used to amplify a single or a few copies of a piece of DNA across the several orders of magnitude in the molecular biology is known as PCR (polymerase chain reaction).
It can generate the thousands of millions of copies of a particular DNA sequence.
A PCR is a cheap and easy tool to amplify a focused segment of DNA. It is very useful tool for the monitoring and diagnosing of genetic diseases, also we can use it for studying the function of targeted segment, identification of criminals, etc. In 1983 Mr. Kary Mullis develops the polymerase chain reaction.

Now, this technique is very common and often indispensable. It supports the variety of applications in medical and biological research labs like the detection and diagnosis of infectious diseases, the diagnosis of hereditary diseases, the identification of genetic fingerprints, functional analysis of genes, DNA-based phylogeny and DNA cloning for sequencing.We are Manufacturer and Supplier of PCR Workstation.

Applications:

The main applications of PCR is divided into following parts:

Basic Research:

  • Bioinformatics
  • Classification of organisms
  • Drug discovery
  • Gene expression studies
  • Genomic cloning
  • Genotyping
  • Molecular Archaeology
  • Molecular Ecology
  • Molecular Epidemiology
  • Mutation screening
  • Site-directed mutagenesis

Applied Research:

  • Genetic matching
  • Gene therapy
  • DNA fingerprinting
  • Detection of pathogens

Molecular Identification:

  • Classification of organisms
  • Detection of pathogens
  • DNA fingerprinting
  • Drug discovery
  • Genetic matching
  • Genotyping
  • Molecular Archaeology
  • Pre-natal diagnosis
  • Molecular Ecology
  • Molecular Epidemiology
  • Mutation screening

Genetic Engineering:

  • Gene expression studies
  • Site-directed mutagenesis

Sequencing:

  • Human Genome Project
  • Genomic cloning
  • Bioinformatics

PCR stages:

We can divide the PCR process into fillowing stages:

Off Stage Leveling:

As the DNA polymerase loses activity, the reaction goes slow, when the consumption of reagents like primers and dNTPs causes them to become limiting.

Exponential amplification:

The amount of product is doubled at every cycle. It is very sensitive, and take only minute quantities of DNA to be present.

Plateau:

Due to exhaustion of reagents and enzyme, no more product accumulates.

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